BIOTECHNOLOGY & BIOINFORMATICS (MPY 102) SYLLABUS

BIOTECHNOLOGY & BIOINFORMATICS (MPY 102) SYLLABUS

1. Genetics: Structure & Function of DNA, DNA Replication & Repair, Expression of Genetic Information: Structure & Function of RNA, Transcription, Genetic code, Translation, Post translational modification.

2. Recombinant DNA Technology: Constructing Recombinant DNA molecules Restriction enzymes, Vectors, Gene Cloning, Genomic libraries, Polymerase Chain reaction – based DNA cloning, Restriction mapping, Blotting techniques, DNA sequencing, Pharmaceutical applications of recombinant DNA.

3. Gene Therapy: General Introduction, Potential target diseases for Gene therapy, Gene transfer methods, Clinical studies, Pharmaceutical production & Regulation.

4. Basics of Immunology, Monoclonal antibodies & Hybridoma technology & its Applications. Vaccines – Conventional vaccines, Modern Vaccine technologies, Genetically improved live vaccines, Genetically improved subunit vaccines, Pharmaceutical considerations.

5. Fundamentals of Cell biology: Cell organization and plasma membrane: Transport of

substances across the membrane. Cellular reproduction: The Cell cycle, Mitosis & Meiosis,

Apoptosis. Cell Signaling: Communication between cells and their environment

6. Molecular biology of cancer: Causes of Cancer & Genetics of Cancer, New strategies for combating cancer.

7. Molecular, Structural and Chemical Biology in pharmaceutical research: Molecular biology of disease and invivo transgenic models, Genomic protein targets and recombinant therapeutics, Structural biology and rational drug design, Chemical biology and Molecular diversity, Gene therapy & DNA/ RNA targeted therapeutics. Future of pharmaceutical

research.

8. Introduction to Bioinformatics: Biological databases, Sequence analysis, Protein structure, Genetic and physical mapping, Application of bioinformatics in pharmaceutical industries.

9. Biostatistics – Graphical representation of Data, Descriptive statistics, Normal distribution, Probability distribution, Sampling & Sampling plans.

Recommended Readings

1. Lehninger ., Principles of Biochemistry

2. Karp, G.,Cell & Molecular Biology.

3. Crommelin, D.J., A., and Sindelar R.D., Pharmaceutical

Biotechnology.

4. Templeton N.S., and Lasic. D.D., Gene Therapy.

5. Benjamin Lewin, Genes.

6. Watson and Trooze, Recombinant DNA Techniques

7. Lesk., Introduction to Bioinformatiics.

8. Watson, Molecular Biology of cell.

9. Old and Primrose, Principles of Gene Manipulations.

10. Watson, J.D., Gilman, M., Recombinant DNA Technology

11. Baxevanis, A.D., Frana, Duelette, B.F.,Bioinformatics

12. Alberts, B., Johnson, A., Lewin, J., Raff, M., Roberts, K., Walter, P.,

molecular biology of the cell

13. Paul, W.E, Fundamentals of Immunology

14. Klug, W.S., Cummings, M.R., Essentials of Genetics

15. Glick, B.R., Pasternak, J.J., Molecular Biotechnology

16. Walker, J.M., Ripley, R., Molecular biology and Biotechnology

17. Bolton, S., Pharmaceutical Statistics.